Tissue Fixation Tissues are fixed: •To preserve cells and tissues constituents •To prevent their degradation Bouin’s fixative/formalin for 24 hrs (with fixative’s volume 20 times greater) 6. Wash in tap water, rinse in distilled 3 changes. 1. To Outline the Proper Procedures for Histopathology . Deparaffinize and hydrate to distilled water. 4. To outline the proper procedures for collecting, preserving, identifying, and processing tissue specimens for evaluation by light microscopic histopathological evaluation. The lab examines tissue materials, based on which an emergency diagnosis is needed during surgery (i.e. 1. 9.1: Rotary Microtome Fig. Laboratory Procedure Manual on histopathology – Mohs surgery and modify or rewrite to conform with the practices in your laboratory. 6. HISTOLOGY AND CYTOLOGY 51 MODULE Microtome Histology and Cytology Notes Fig. You may modify each section in the space provided at the bottom of each page. PROCEDURE: 1. A number of histologic stains are available that are routinely used to visualize fungi in tissue sections. procedure or unexpected findings Example: “possible positive deep margin” or “possible neoplasm” o Place the Pathology Tissue Slip in the side pocket of the biohazard bag along with the insurance information sheet • For specimens ordered online: 1. Wash in tap water, rinse in distilled. Hence, the book is titled as Histopathology Techniques and its Management. histopathology techniques. 9 HISTOPATHOLOGY LABORATORY Histopathology laboratory is a unit performing clinical activity associated with diagnosis during and post-surgery. Histology Procedure Fixation Tissue Processing a. Dehydration b. This textbook deals with basic histopathology techniques: Introduction and examination of tissue, fixation and fixatives, tissue processing and … II. Histology Procedure Fixation 5. RESPONSIBILITY . histopathology is the only way to diagnose infections caused by L. loboi or Rhinosporidium seeberi [Figure 2] as cultivation techniques have to date been unsuccessful. a. 2. 5. I. 18 Record of Changes Clearing c. Infilteration 7. 5.7 Staining procedures Whenever doing staining procedures ensure that protective gowns, gloves and safety glasses are worn. 3. 1% Sodium metabisulfite, 1 minute, room temperature. *2% Chromic acid, microwave Hi power, 45 seconds, allow to stand for 5 minutes. When ‘bringing sections to water’ or ‘dehydrating, clearing and mounting’ always HISTOLOGY ATC BRANCH HD-19-HSC-10.2 HISTOLOGY SPECIMEN COLLECTION Effective: 2/18/13, Rev 3/4/2014 9. Choose appropriate inpatient surgical request card or outpatient histopathology specimen requisition and fill in completely (if applicable). 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